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9dc0f7f061%In this study we investigated the role of TCF1 in reversing T cell exhaustion by CRISPR-HDR technology.
c39d71d554%The aim of this study is to investigate the role of all human TFs and coTFs in the status of six PDAC cell lines
51d8ca5750%The aim of this study is to investigate the role of all human TFs and coTFs in the status of six PDAC cell lines
4e217ccd45%CRISPR-based genetic perturbation screens have revolutionized the ability to link genes to cellular phenotypes with unprecedented precision and scale. However, conventional pooled CRISPR screens requi…
cab7d9ff40%We tested the essentiality of PDA state-specific Master Regulators in vivo by transducing a sgRNA library targeting 93 regulatory proteins into Cas9 expressing KP4 and HPAFII human cell lines. Cells w…
a4f2d85a38%Chromatin accessibility at LTR12C family has been shown to b required to maintain stemness properties in leukemic stem cells. Here using CRISPRi epigenetic silencing strategy, we targetted LTR12C fa…
a38e558238%Comparison of transcriptional profiling of isolated T cells of healthy individuals after treatment with supernatant of peripheral blood mononuclear cells (PBMCs) of the same individuals treated overni…
dc5f68ab36%The aim of this study is to investigate the effect of the combinatorial overexpression of the top GLS MRs in a MOS cell line (at single-cell level).
c1b61c6436%We identify the endogenous lipid 9-PAHSA as a potent enhancer of T cell anti-tumor function and stemness, validated in murine models and a clinical trial. Mechanistically, we identify ICAM2 as a direc…
b97c4cdb35%To assess the impact of B cell deficiency on MCMV-specific CD8⁺ T cells, equal numbers of M45 tetramer-positive CD8⁺ T cells were sorted from wild-type and B cell–deficient JHT mice. The isolated anti…
8182ae8834%To identify upregulated gene sets and pathways enriched by GeCas12a2 activation.
5cc5182233%Manipulating epigenetic layers during transcriptional regulation poses significant challenges, yet advancements in dCas-based systems offer promising avenues for targeted manipulation. DNA methylation…
9ab65b7633%Manipulating epigenetic layers during transcriptional regulation poses significant challenges, yet advancements in dCas-based systems offer promising avenues for targeted manipulation. DNA methylation…
3aaeb96033%Utilising adeno-associated virus (AAV) to deliver CRISPR/Cas has enhanced the generation of knock-out mice, but single-AAV delivery of Cas and homology-directed-repair (HDR) templates in livestock rem…
1f0fde2633%Utilising adeno-associated virus (AAV) to deliver CRISPR/Cas has enhanced the generation of knock-out mice, but single-AAV delivery of Cas and homology-directed-repair (HDR) templates in livestock rem…
a33ec72633%In this study, we treated PBMCs of 6 healthy individuals with sCD14 overnight. The day after, the supernatant of treated PBMCs was collected and isolated T cells from the same individuals were stimula…
2970ba4c33%The number variations and specific roles of regulatory T (Treg) cells within spinal cord injury (SCI) remain elusive. To further investigate T cell community chages post-SCI, we conducted the first si…
28f71e1a32%Direct reprogramming of somatic cells into neurons offers a promising strategy for studying neurological disorders and developing cell-based therapies. This protocol describes the 3D direct reprogramm…
b512310e32%In this study the top Lineage Master Regulators of PDA were overexpressed in the KP4 Morphogenic cell line to study their reprogramming effect
bf11c0f632%Manipulating epigenetic layers during transcriptional regulation poses significant challenges, yet advancements in dCas-based systems offer promising avenues for targeted manipulation. DNA methylation…