urn:osa:lingual.bio:rec:9299263f-e95f-4efb-b895-62705fd58b3e@1Spatially resolved m6A profiling using m6A-ARTR-DBiT
Methylation profiling by high throughput sequencing; Expression profiling by high throughput sequencingSummary
N6-methyladenosine (m6A) on RNA plays diverse regulatory roles, yet its spatial distribution within tissues remains largely unexplored. Here, we develop a spatial m6A profiling assay leveraging reverse transcription-based detection and deterministic barcoding in tissue (m6A-ARTR-DBiT), enabling spatially resolved sequencing of m6A within the native tissue context. Application of m6A-ARTR-DBiT to embryonic and adult mouse brain generated high-resolution whole transcriptome spatial m6A maps, offering insights into region-specific m6A modification.
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urn:osa:lingual.bio:rec:9299263f-e95f-4efb-b895-62705fd58b3e@1